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elisa试剂盒_森贝伽自主品牌

关于森贝伽
森贝伽生物依托国内外技术研发人才的优势,由留美资深科学家与数位国内多年从事生物领域研究的博士、硕士等高校生物领域研究人员共同创立,在科学研究和医疗检测领域享有极高的声誉。
elisa试剂盒-森贝伽自主品牌
拥有自己的实验室与研发基地,专业的技术研发团队, 长期致力于ELISA试剂盒、生物试剂的研发,拥有10年研发经验。
产品图片elisa试剂盒图片elisa试剂盒图片
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1、Haijun Liu,Shencun Fang,Wei Wang,etc.Macrophage-derived MCPIP1 mediates silica-induced pulmonary fibrosis via autophagy[J].Part Fibre Toxicol.2016:1-17.

作者单位:东南大学

使用产品:Human MCP-1, TNF-α, TGF-β ELISA kit

2、Juan Zhang,Meng Cao,Wenwen Yang,etc.Inhibition of Glucose-6-Phosphate Dehydrogenase Could Enhance 1,4-Benzoquinone-Induced Oxidative Damage in K562 Cells[J].Oxid Med Cell Longev.2016:1-11.

作者单位:东南大学

使用产品:Human NADP, NADPH ELISA Kit

3、Qian-NanDi,Wei-XinCao,RunXu,etc.Chronic low-dose exposure of nonylphenol alters energy homeostasis in the reproductive system of female rats[J].Toxicology and Applied Pharmacology.2018,1(348):67-75.

作者单位:东南大学

使用产品:Rat E2 ELISA Kit

4、Muhammad Noman Khan,Bing Wang,Jing Wei,etc.CXCR1/2 antagonism with CXCL8/Interleukin-8 analogue CXCL8(3-72)K11R/G31P restricts lung cancer growth by inhibiting tumor cell proliferation and suppressing angiogenesis[J].Oncotarget.2015,6(25): 21315-21327.

作者单位:国立清华大学(台湾)

使用产品:Human CXCL1, CXCL6, CXCL8 ELISA kit

5、Cheng Yu Wang,Xiang Dan Li,Zhi Hong Hao,etc.Insulin-like growth factor-1 improves diabetic cardiomyopathy through antioxidative and anti-inflammatory processes along with modulation of Akt/GSK-3β signaling in rats[J].Korean J Physiol Pharmacol.2016,20(6): 613-619.

作者单位:延边大学

使用产品:Rat IL-1β, TNF-a ELISA kit

6、Xiaoyue Shen,Wenkui Sun,Yi Shi,etc.Altered Viral Replication and Cell Responses by Inserting MicroRNA Recognition Element into PB1 in Pandemic Influenza A Virus (H1N1) 2009[J].Mediators Inflamm.2015:1-12.

作者单位:南京大学

使用产品:Human IL-6 ELISA kit

7、Chun‐Tao Lei,Hua Su,Chen Ye,etc.The classic signalling and trans-signalling of interleukin‐6 are both injurious in podocyte under high glucose exposure[J].J Cell Mol Med.2018,22(1):251-260.

作者单位:华中科技大学

使用产品:Human sIL‐6R, sgp130 ELISA kit

8、Jian’an Bai,He Na,Xiumei Hua,etc.A retrospective study of NENs and miR-224 promotes apoptosis of BON-1 cells by targeting PCSK9 inhibition[J].Oncotarget.2017,8(4): 6929-6939.

作者单位:南京医科大学附属逸夫医院

使用产品:Human glucocorticoid ELISA kit

9、Zhan Zhang, Di Wang,Shanlei Qiao,etc.Metabolic and microbial signatures in rat hepatocellular carcinoma treated with caffeic acid and chlorogenic acid[J].Scientific reports.2017(7):1-10.

作者单位:南京医科大学

使用产品:Rat LPS ELISA kit

10、Meng-Chen Lu,Jian-Ai Ji,Yong-Lin Jiang,etc.An inhibitor of the Keap1-Nrf2 protein-protein interaction protects NCM460 colonic cells and alleviates experimental colitis[J].Scientific reports. 2016(6):1-13.

作者单位:中国药科大学

使用产品:Human IL-1β, IL-6, TNF-α, IFN-γELISA kit

11、Xiaotong Sun,Jing Wei,Yawei Tang,etc.Leptin-induced migration and angiogenesis in rheumatoid arthritis is mediated by reactive oxygen species[J].FEBS Open Bio.2017,7(12):1899-1908.

作者单位:大连医科大学

使用产品:Human VEGF, IL‐1β, TNF-α ELISA kit

12、Geng Tian,Linlin Cheng,Xuewei Qi,etc.Transgenic Cotton Plants Expressing Double-stranded RNAs Target HMG-CoA Reductase (HMGR) Gene Inhibits the Growth, Development and Survival of Cotton Bollworms[J].Int J Biol Sci. 2015,11(11):1296-1305.

作者单位:华中农业大学

使用产品:Plant HMGR ELISA kit 

13、Siyuan Cui,Lili Men,Yu Li,etc.Selenoprotein S Attenuates Tumor Necrosis Factor-α-Induced Dysfunction in Endothelial Cells[J].Mediators Of Inflamm.2018:1-16.

作者单位:大连医科大学附属第一医院

使用产品:Human ICAM-1, VCAM-1 ELISA kit

14、Peng-Tao Ren,Yuan Zhang,etc.Comparative investigation of the effects of specific antigen-sensitized DC-CIK and DC-CTL cells against B16 melanoma tumor cells[J].Mol Med Rep. 2017,15(4):1533-1538.

作者单位:河北医科大学第二医院

使用产品:Human CCL19, CCL22 ELISA kit

15、Ming Ma,Lian-mei Zhao,Xing-xiao Yang,etc.p-Hydroxylcinnamaldehyde induces the differentiation of oesophageal carcinoma cells via the cAMP-RhoA-MAPK signalling pathway[J].Scientific reports.2016(6):1-13.

作者单位:河北医科大学第四医院

使用产品:Human cAMP, CEA, SCC ELISA kit

16、Yucai Chen,Tianyi Yuan,Huifang Zhang,etc.Activation of Nrf2 Attenuates Pulmonary Vascular Remodeling via Inhibiting Endothelial-to-Mesenchymal Transition: an Insight from a Plant Polyphenol[J].Int J Biol Sci.2017,13(8):1067-1081.

作者单位:中国医学科学院中国协和医科大学药物研究所

使用产品:Human MDA ELISA kit

17、Rao Sun,Zuoxia Zhang,Yishan Lei,etc.Hippocampal activation of microglia may underlie the shared neurobiology of comorbid posttraumatic stress disorder and chronic pain[J].Mol Pain. 2016(12):1-13.

作者单位:南京大学医学院鼓楼医院

使用产品:Human IL-1β, TNF-α ELISA kit

18、Yuanyuan Zhuang,Jiabao Liu,Pei Ma,etc.Tamarixinin A Alleviates Joint Destruction of Rheumatoid Arthritis by Blockade of MAPK and NF-κB Activation[J].Front Pharmacol.2017(8):1-11.

作者单位:北京协和医学院

使用产品:Mouse CII antibody ELISA kit

19、Huawen Shi,Guangchao Yang,Tongsen Zheng,etc.A preliminary study of ALPPS procedure in a rat model[J].Scientific reports. 2015(5):1-12.

作者单位:哈尔滨医科大学附属第一医院

使用产品:Rat NF-KB ELISA kit

20、Bo Qiu,Ming Gong,Qi-Ting He,etc.Controlled Release of Interleukin-1 Receptor Antagonist from Hyaluronic Acid-Chitosan Microspheres Attenuates Interleukin-1β-Induced Inflammation and Apoptosis in Chondrocytes[J].Biomed Res Int.2016:1-12.

作者单位:武汉大学人民医院

使用产品:Rat GAG ELISA kit

21、Gairu Li,Yang Xiang,Jin Zhao,etc.Saccharum Alhagi polysaccharide-1 and -2 promote the immunocompetence of RAW264.7 macrophages in vitro[J].Exp Ther Med.2018,15(4): 3556-3562.

作者单位:新疆医科大学

使用产品:Mouse IL-2, IL-1β, TNF-α ELISA kit

22、Yuanyuan Zhang,Minhui Sun,Yuwei Han,etc.The saponin DT-13 Attenuates Tumor Necrosis Factor-α-induced Vascular Inflammation Associated with Src/NF-кB/MAPK Pathway Modulation[J].Int J Biol Sci.2015,11(8):970-981.

作者单位:中国药科大学

使用产品:Mouse ICAM-1 (sICAM-1), VCAM-1 (sVCAM-1) ELISA kit

23、Ying Huang,Shaofeng Wang,Chunya Zhang,etc.Experimental study of the anti-atherosclerotic effect of demethylzeylasteral[J].Exp Ther Med.2017,13(6): 2787-2792.

作者单位:上海市人民医院

使用产品:Rabbit anti-oxidized LDL (ox-LDL) antibody  ELISA kit

24、Lin Li,Ting Xiao,Fang Li,etc.Hydrogen sulfide reduced renal tissue fibrosis by regulating autophagy in diabetic rats[J].Mol Med Rep.2017,16(2): 1715-1722.

作者单位:华南大学第一附属医院

使用产品:Rat UP ELISA kit

25、Yong Zhang,Changyin Li,Hu Li,etc.miR-378 Activates the Pyruvate-PEP Futile Cycle and Enhances Lipolysis to Ameliorate Obesity in Mice[J].EBioMedicine.2016(5): 93-104.

作者单位:中国医学科学院

使用产品:Mouse Insulin, FFA ELISA kit

26、Haitao Zou,Ruixue Su,Jing Ruan,etc.Toll-like receptor 3 pathway restricts Marek’s disease virus infection[J].Oncotarget.2017,8(41):70847-70853.

作者单位:扬州大学

使用产品:Chicken IFN-β ELISA kit

27、Na Yang,Run-bin Sun,Xing-long Chen,etc.In vitro assessment of the glucose-lowering effects of berberrubine-9-O-β-D-glucuronide,an active metabolite of berberrubine[J].Acta Pharmacol Sin.2017,38(3): 351-361.

作者单位:中国药科大学

使用产品:Rat α- glucosidase ELISA kit

28、Xiaoxiao Qian,Juan Tan,Ling Liu,etc.MicroRNA-134-5p promotes high glucose-induced podocyte apoptosis by targeting bcl-2[J].Am J Transl Res.2018,10(3): 989-997.

作者单位:南京医科大学附属第二医院

使用产品:Mouse microalbuminuria ELISA Kit

29、Ruiping Fan,Yuwei Han,Han Han,etc.DT-13 ameliorates TNF-α-induced nitric oxide production in the endothelium in vivo and in vitro[J].Biochemical and Biophysical Research Communications.2018,1(495):1175-1181.

作者单位:中国药科大学

使用产品:Mouse nitric oxide, IL-8, MCP-1, ROS, TNFR ELISA kit

30、Yu Li,Ning Hao,Suping Zou,etc.Immune Regulation of RAW264.7 Cells In Vitro by Flavonoids from Astragalus complanatus via Activating the NF-κB Signalling Pathway[J].Journal of Immunology Research.2018:1-9.

作者单位:安徽农业大学

使用产品:Mouse IL-1β, IL-6, TNF-α, NO ELISA Kit

31、Xichun Wang,Shibin Feng,Nana Ding,etc.Anti-Inflammatory Effects of Berberine Hydrochloride in an LPS-Induced Murine Model of Mastitis[J].Evidence-Based Complementary and Alternative Medicine,2018:1-9.

作者单位:安徽农业大学

使用产品:Mouse TNF-α, IL-6, and IL-1β ELISA Kit

32、JieChao,YanLv,JinChen,etc.SiO2-induced release of sVEGFRs from pulmonary macrophages[J].Respiratory Physiology & Neurobiology,2018(247):1-8.

作者单位:东南大学

使用产品:Human sVEGFR ELISA Kit

33、Meng-Chen Lu, Qiong Jiao,Tian Liu,etc.Discovery of a head-to-tail cyclic peptide as the Keap1-Nrf2 protein-protein interaction inhibitor with high cell potency[J].European Journal of Medicinal Chemistry.2018,1(143):1578-1589.

作者单位:中国药科大学

使用产品:Mouse IL-1β, IL-18, TNF-α ELISA Kit

34、Zhengfeng Wu,Tianhong Gao,Ronglign Zhong,etc.Antihyperlipidaemic effect of triterpenic acid-enriched fraction from Cyclocarya paliurus leaves in hyperlipidaemic rats[J].Pharmaceutical Biology,2017,1(55):712-721.

作者单位:中国药科大学

使用产品:Rat apoB48 ELISA Kit

35、Rao Sun,Zhibin Zhao,Jiying Feng,etc.Glucocorticoid-Potentiated Spinal Microglia Activation Contributes to Preoperative Anxiety-Induced Postoperative Hyperalgesia[J].Mol Neurobiol.2017,54(6):4316-4328.

作者单位:南京鼓楼医院

使用产品: Rat CS, IL-1β, TNF-α ELISA Kit

36、Guoxiang You, Peifang Wang, Jun Hou,etc.Insights into the short-term effects of CeO2 nanoparticles on sludge dewatering and related mechanism[J].Water Research.2017,04(011):93-113.

作者单位:河海大学

使用产品:Plant LAP ELISA Kit

37、Peifang Wang, Guoxiang You, Jun Hou,etc.Responses of wastewater biofilms to chronic CeO2 nanoparticles exposure: Structural, physicochemical and microbial properties and potential mechanism[J].Water Research.2018:208-217.

作者单位:河海大学

使用产品:Plant AHL ELISA Kit

38、Yi Xu, Chao Wang, Jun Hou,etc.Strategies and relative mechanisms to attenuate the bioaccumulation and biotoxicity of ceria nanoparticles in wastewater biofilms[J].Bioresource Technology.2018:102-109.

作者单位:河海大学

使用产品:Plant ATP, DHA ELISA Kit

39、Junsheng Liu,Zhennan Gu,Wenwei Lu,etc.Multiple mechanisms applied by Lactobacillus pentosus AT6 to mute the lethal effects of Salmonella in a mouse model[J].Food Funct.2018, 23;9(5):2787-2795.

作者单位:江南大学

使用产品:Mouse CRP, LPS ELISA Kit

☆☆☆☆☆需要elisa实验代做的请点击进入elisa代测技术服务
检测目的:用于测定血清,血浆及相关液体等样本。例如适合检测包括血清、血浆、尿液、胸腹水、灌洗液、脑脊液、细胞培养上清、组织匀浆等标本。
森贝伽供应的种属:人、大鼠、小鼠、豚鼠、兔子、猪犬、牛羊、鸡鸭、植物ELISA试剂盒等
售后技术:公司提供全方位的售前、售中、售后技术支持,为您解决实验过程中遇到的问题
客户案例:凭借着森贝伽实验室先进的技术平台和专业的售后技术服务,此期间我们已为全国众多医疗机构、重点高校实验室及研究所提供了ELISA产品及ELISA代测服务,深受各高校实验室和医院的师生欢迎。
森贝伽ELISA试剂盒组成:

产品组成

48孔配置

96孔配置

Storage

标准品:180ng/L

0.5ml×1瓶

0.5ml×1瓶

2-8℃

标准品稀释液

1.5ml×1瓶

1.5ml×1瓶

2-8℃

酶标试剂

3ml×1瓶

6ml×1瓶

2-8℃

样品稀释液

3ml×1瓶

6ml×1瓶

2-8℃

显色剂A液

3ml×1瓶

6ml×1瓶

2-8℃

显色剂B液

3ml×1瓶

6ml×1瓶

2-8℃

终止液

3ml×1瓶

6ml×1瓶

2-8℃

浓缩洗涤液

(20ml×20倍)×1瓶

(20ml×30倍)×1瓶

2-8℃

酶标包被板

1×48

1×96

2-8℃

封板膜

2片

2片

RT

密封袋

1个

1个

RT

说明书

1份

1份

RT


操作步骤:

elisa试剂盒操作步骤

注意事项:
1.试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存。
2.浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果。
3.各步加样均应使用加样器,并经常校对其准确性,以避免试验误差。一次加样时间最好控制在5分钟内,如标本数量多,推荐使用排枪加样。
4.请每次测定的同时做标准曲线,最好做复孔。如标本中待测物质含量过高(样本OD值大于标准品孔第一孔的OD值),请先用样品稀释液稀释一定倍数(n倍)后再测定,计算时请最后乘以总稀释倍数(×n×5)。
5.封板膜只限一次性使用,以避免交叉污染。
6.底物请避光保存。
7.严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.
8.所有样品,洗涤液和各种废弃物都应按传染物处理。
9.本试剂不同批号组分不得混用。
10.如与英文说明书有异,以英文说明书为准。
标准曲线的绘制方法:
可以采用各种绘图软件来绘制ELISA标准曲线,我公司以“Curve Exert1.3”软件为例,绘制的ELISA标准曲线如下:

 ELISA标准曲线

计算:以标准物的浓度为横坐标,OD值为纵坐标,在坐标纸上绘出标准曲线,根据样品的OD值由标准曲线查出相应的浓度;再乘以稀释倍数;或用标准物的浓度与OD值计算出标准曲线的直线回归方程式,将样品的OD值代入方程式,计算出样品浓度,再乘以稀释倍数,即为样品的实际浓度