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SBJ-0288

Masson三色染色液

规格 结缔组织染色
  • 英文名:
  • Masson trichrome stain Kit
  • CAS号:
  • 分子式:
  • 品牌:
  • Sbjbio
  • MDL:
  • 储存条件:
  • RT,避光,12个月
产品编号 销售价促销价 库存 数量 单位 加入购物车
SBJ-0288-6*50ml ¥300.00元 ¥150.00元 准现货 EA 加入购物车
SBJ-0288-6*100ml ¥560.00元 ¥280.00元 准现货 EA 加入购物车

商品描述

文献引用

质检证书(COA)

相关商品

Masson三色染色试剂盒说明书

【产品组成】

Masson三色染色试剂盒是由Weigert染液A、Weigert染液B、酸性乙醇分化液、Masson蓝化液、丽春红品红染色液、弱酸溶液、磷钼酸溶液、苯胺蓝染色液组成

【产品概述】

结缔组织狭义上是指其含有的三种纤维:胶原纤维、网状纤维、弹力纤维,而胶原纤(collagenfiber)是分布最广、含量最多的一种纤维。Masson三色染色又称马松染色,是结缔组织染色中最经典的一种方法,是胶原纤维染色权威而经典的技术方法。所谓三色染色通常是指染胞核和能选择性的显示胶原纤维和肌纤维。该法染色原理不阴离子染料分子的大小和组织的渗透有关:分子的大小由分子量来体现,小分子量易穿透结构致密、渗透性低的组织;而大分子量则只能进入结构疏松的、渗透性高的组织。然而,淡绿或苯胺蓝的分子量都很大,因此Masson染色后肌纤维呈红色,胶原纤维呈绿色(淡绿)或蓝色(苯胺蓝),主要用于区分胶原纤维和肌纤维。染色稳定;分化时间短,1~2s;色彩清晰鲜艳;适用范围广,适宜于组织的石蜡切片、冰冻切片等染色;所染切片保存时间长且不易褪色。

【使用方法】

1、切片常规脱蜡至水,用配制好的Weigert铁苏木素染色5~10min。

2、用酸性乙醇分化液分化,水洗。

3、用Masson蓝化液返蓝,水洗。

4、蒸馏水洗1min。

5、丽春红品红染色液染色5~10min。

6、在上述操作过程中按蒸馏水:弱酸溶液=2:1比例配制弱酸工作液,用弱酸工作液洗1min。

7、磷钼酸溶液洗1~2min。

8、用配制好的弱酸工作液洗1min。

9、直接入苯胺蓝染色液中染色1~2min。

10、用配制好的弱酸工作液洗1min。

11、95%乙醇快速脱水。无水乙醇脱水3次,每次5~10s。

12、二甲苯透明3次,每次1~2min。中性树胶封固。

【染色结果】

细胞核,胶原纤维/蛋白

蓝色

胞浆、肌肉、红细胞

红色

【注意事项】

1、切片脱蜡应尽量干净。固定起着重要的作用,使用不同的固定液可延或缩短染色时间。

2、取A1、A2等量混合即为Weigert铁苏木素染液,一般24h失去染色力。

3、酸性乙醇分化时间应根据切片厚薄、组织的类别和新旧而定。

4、弱酸溶液可使色彩更清晰鲜艳,如使用量大可自行配制0.1~0.3%乙酸溶液予以替代。

5、磷钼酸分化时要在镜下控制,分化到胶原纤维呈淡红色、纤维呈红色即可。分化时间根据染色深浅而定,一般1~2min。

6、Masson蓝化液亦可自行配制Scott促蓝液或0.1~1%碳酸锂水溶液予以替代。 

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