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C11330500BT

D-MEM/F-12 培养基 1:1,含15mM HEPES缓冲液和L-谷氨酰胺

规格 含15mM HEPES缓冲液和L-谷氨酰胺
  • 英文名:
  • DMEM/F-12, HEPES
  • CAS号:
  • 分子式:
  • 品牌:
  • ThermoFisher
  • MDL:
  • 储存条件:
  • 2-8℃
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C11330500BT-500ml 含15mM HEPES缓冲液和L-谷氨酰胺 ¥101.00元 ¥70.70元 准现货 EA 加入购物车

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引用及参考文献

质检证书(COA)

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描述

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. 


This DMEM/F-12 is modified as follows:

With

• L-glutamine

• HEPES

• Phenol Red


Using DMEM/F-12

DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system

DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM/F-12 product made in our Scotland facility (31330-038). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


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